Step by Step Guide

TBSS (voxel-wise analysis of local diffusion measures)

• Do a tensor fit with DTIFIT

• Make a directory for the analysis
• Copy the FA images into this directory

• Standard TBSS analysis (of FA):

  • tbss_1_preproc *.nii.gz

  • tbss_2_reg -T (recommended - one registration per image, deriving a study-specific template)

    • alternative is to use a custom template (-t)
    • use best target from all images (-n) - lots of registrations

  • tbss_3_postreg -S (-T for FMRIB58)

    • view/check the skeleton output

  • tbss_4_prestats 0.2

  • cd stats

  • Use the Glm GUI to create appropriate design matrices and contrasts, and save the design

  • Run randomise with the design files from the Glm GUI and appropriate multiple-comparison correction (e.g. --T2)

• View the results
  • look at the *corrp* images (these are the multiple comparison corrected p-values, stored as 1-p values)
  • tbss_fill for making a thicker skeleton for more easy viewing and creating figures for publications